Inhibitory effect of AuNPs-Endostar on melanoma lung metastasis in mice

AIM: To observe the influence of gold nanoparticles combined with Endostar (AuNPs-Endostar) on the melanoma lung metastasis of mice and the underlying mechanism. METHODS: C57BL/6 mice (n=24) were selected for constructing the model of spontaneous lung metastasis of melanoma B16-F10 cells. Subsequently, the mice were randomly divided into Endostar group, AuNPs group, AuNPs-Endostar group and model group. After the formation of melanoma, the mice in each group were injected with different drugs through tail vein for 0.1 mL daily. After 9 d, the mice were narcotized for cutting the tumors in situ. After the operation, they were raised for 2 weeks before killed for obtaining the lung tissues to observe the situation of the metastasis. HE staining was utilized for observing the necrosis status of the tumors in situ, while immunostaining was applied for testing the expression of CD31, carbonic anhydrase-IX (CA-IX), vimentin and zonula occludens-1 (ZO-1) in the tumors. RESULTS: Compared with model group, the pulmonary metastasis in the groups with medical treatment was obviously reduced. In AuNPs-Endostar group, the metastasis inhibition rate was the highest, and the tumor necrosis was also decreased obviously, with the significant reduction of CD31, CA-IX and vimentin expression in the tumors and significant increase in ZO-1 expression. CONCLUSION: Compared with using Endostar or AuNPs alone, the combination of AuNPs with Endostar significantly improves the curative effect of inhibiting the pulmonary metastasis of melanoma in the mice. The mechanism may be related to reducing the tumor angiogenesis, norma-lizing the blood vessels and improving tumor hypoxia, thus inhibiting the tumor epithelial-mesenchymal transition, increasing the tight junctions between tumor cells and decreasing the invasiveness.     

过渡乳营养价值和对犊牛生产性能影响的研究进展

过渡乳是初乳向常乳过渡的乳汁。目前国内对于过渡乳的概念还没有深刻的认识。根据调研,目前在犊牛灌服初乳后,为了节省人力、节约成本,牧场对于过渡乳的使用并不充分,有的完全废弃,有的违规操作进行售卖,而采用代乳粉、常乳等代替过渡乳对犊牛进行饲喂。针对这一问题,本文从过渡乳的定义、营养价值和对犊牛生产性能的影响三个方面进行综述,以为过渡乳的使用以及犊牛7日龄内饲喂方案的制定提供参考。     

THE DEVELOPMENT OF ALUMINIUM MATRIX MICRO -GLASSBALL COMPOSITE AND THE ANALYSIS ON ITS FORMING MECHANISMS

The problem that the micro-glassball is not wettable in molten aluminium alloys can be solved by a special technology that is different from rheocasting and squeezecasting methods. The metten metal can fill the cavity of the glass ball through the broken place on its wall, and an original micro -structure will be formed. This material has good comprehensive mechanical proper ties and this method can be used to other kinds of ceramic powders. The mechanisms of the formation of this materials have been discussed as well.     

The tyranny of taste: the case of organic rice in Cambodia

Fair-trade and organic products are often sold at price premiums justified by smaller production volumes that are associated with greater social and environmental responsibility. The consumption of these products confers on the consumer a greater sense of morality – and usually a claim to better taste. This paper tells the story of attempts to promote organic/fair-trade rice production by de facto organic Cambodian farmers for export to North American and European markets in order to assist poor farmers to trade their way out of poverty. It demonstrates that instead of promoting sustainable agriculture and fair trade between developed and developing markets, organic/fair-trade projects may impose First World consumer ideals and tastes that are out of step with the larger realities of agrarian transition in Cambodia and the wider region of developing Southeast Asia.     

DYNAMIC ANALYSIS OF ASYMMETRIC FRAME-SHEAR WALL SYSTEM BY STAGE CONTINUITY METHOD

The lateral structures of frame-shear wall system are simplified into bending beams and shear beams which stiffness can vary in different stages along the building height. The three-dimension stiffness matrix and mass matrix are deduced, and the characteristic equation is established to calculate the tortuous free vibration of the asymmetric system. The method is simple and has satisfactory precision, and it is easily fulfilled in micro-computers.     

Metapositive Definite Problem in Both Kronecker Product and Hadamard Product of Metafositive Definit Matrices

In this paper,a lass of particular metapositive difinite matrices has beendissussed,and two different sufficet conditions under which Hadamard self-multiplications andKronecker self-multiplications of a metapositive difinite matrix are also metapositive difinitematrices have been given.     

辣椒秸秆复合基质对西瓜育苗质量的影响

为降低西瓜基质育苗生产成本,采用不同比例的辣椒秸秆与蛭石、珍珠岩进行复配,探讨其对西瓜幼苗形态建成及生理特性的影响,以明确最佳的草炭替代材料。试验设置L1(V_辣椒秸秆∶V_蛭石∶V_珍珠岩=1∶1∶1)、L2(V_辣椒秸秆∶V_蛭石∶V_珍珠岩=2∶1∶1)、L3(V_辣椒秸秆∶V_蛭石∶V_珍珠岩=4∶1∶1)3个处理和对照CK(V草炭∶V_蛭石∶V_珍珠岩=2∶1∶1)。结果表明,L2处理的西瓜幼苗株高、茎粗、叶片数、干鲜质量、根尖数、壮苗指数和根冠比最大,根系活力最高;L1处理的西瓜幼苗出苗率最高,但与L2差异不显著;L3处理的西瓜幼苗根长最大,但与L2差异不显著。L2处理的西瓜幼苗叶片叶绿素a、叶绿素b、叶绿素(a+b)、类胡萝卜素含量和净光合速率最高,较对照分别增加10.98%、11.51%、11.13%、7.71%和21.38%。因此,由辣椒秸秆、蛭石、珍珠岩体积比按2:1:1复合而成的育苗基质可以替代常规草炭育苗基质(V草炭∶V_蛭石∶V_珍珠岩=2∶1∶1)进行西瓜育苗。     

一种优化的基于协同过滤的农业信息推荐系统研究

随着信息爆炸而引发的信息过载和资源迷向的问题日趋严重,特别是对于农民来说,对计算机技术的掌握不多。为了向农民及时、方便、高效地提供其感兴趣的农业信息及知识,许多学者都对推荐系统技术进行了研究。为此,首先介绍了目前推荐系统的关键技术,并针对目前推荐系统中存在的用户评价矩阵稀疏问题提出了通过项目聚类来构造出一个无缺失的用户评价矩阵。考虑到农民行为差异性,在进行评价预测之前先对评分进行标准化处理。     

紫叶矮樱不同基质育苗比较试验

以炉渣、锯末、菇渣废料、鸡粪、羊粪、田园土分别按照不同体积比配制9种供试基质,对紫叶矮樱进行处理,研究不同基质养分对紫叶矮樱苗木株高及成品苗质量的影响.结果表明:G6基质(田园土∶炉渣∶菇渣废料∶鸡粪=6∶3∶0.5∶0.5)为最佳栽培基质.